2017 |2016 |2015 | 2014 | 2013 | 2012 | 2011 | 2010

2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002 | 2001 | 2000

1999 | 1998 | 1996 | 1995 | 1994 | 1993 | 1992 | 1991 | 1989


172) Hutchison CDM, Cordon-Preciado V, Morgan RML, Nakane T, Ferreira J, Dorlhiac G, Sanchez-Gonzalez A, Johnson AS, Fitzpatrick A, Fare C, Marangos JP, Yoon CH, Hunter MS, DePonte DP, Boutet S, Owada S, Tanaka R, Tono K, Iwata S and van Thor JJ.
X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature.
Int J Mol Sci.18(9): 1918 (2017)
DOI: 10.3390/ijms18091918. PubMed ID: 28880248
171) Kubo M, Nango E, Tono K, Kimura T, Owada S, Song C, Mafune F, Miyajima K, Takeda Y, Kohno J, Miyauchi N, Nakane T, Tanaka T, Nomura T, Davidsson J, Tanaka R, Murata M, Kameshima T, Hatsui T, Joti Y, Neutze R, Yabashi M. and So Iwata.
Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA
J. Synchrotron Rad. 24; 1089-1091(2017)
DOI: 10.1107/S160057751701030X. PubMed ID: 28862633
170) Yamashita, K, Kuwabara N, Nakane T, Murai T, Mizohata E, Sugahara M, Pan D, Masuda T, Suzuki M, Sato T, Kodan A, Yamaguchi T, Nango E, Tanaka T, Tono K, Joti Y, Kameshima T, Hatsui T, Yabashi M, Manya H, Endo T, Kato R, Senda T, Kato H, Iwata S, Ago H, Yamamoto M, Yumoto F. and Nakatsu T.
Experimental phase determination with seleno- methionine or mercury-derivatization in serial femtosecond crystallography
IUCrJ. 4(5); 639-647 (2017)
DOI: 10.1107/S2052252517008557. PubMed ID: 28989719
169) Thomaston JL, Woldeyes RA, Nakane T, Yamashita A, Tanaka T, Koiwai K, Brewster AS, Barad BA, Chen Y, Lemmin T, Uervirojnangkoorn M, Arima T, Kobayashi J, Masuda T, Suzuki M, Sugahara M, Sauter NK, Tanaka R, Nureki O, Tono K, Joti Y, Nango E, Iwata S, Yumoto F, Fraser JS, DeGrado WF.
XFEL structures of the influenza M2 proton channel: Room temperature water networks and insights into proton conduction.
PNAS 2017 ; published ahead of print August 23, (2017)
DOI: 10.1073/pnas.1705624114. PubMed ID:28835537
168) Taniguchi R, Inoue A, Sayama M, Uwamizu A, Yamashita K, Hirata K, Yoshida M, Tanaka Y, Kato HE, Nakada-Nakura Y, Otani Y, Nishizawa T, Doi T, Ohwada T, Ishitani R, Aoki J and Nureki O.
Structural insights into ligand recognition by the lysophosphatidic acid receptor LPA6
Nature 548; 356-360 (2017)
DOI: 10.1038/nature23448. PubMed ID:28792932
167) Naitow H, Matsuura Y, Tono K, Joti Y, Kameshima T, Hatsui T, Yabashi M, Tanaka R, Tanaka T, Sugahara M, Kobayashi J, Nango E, Iwata S and Kunishima N.
Protein-ligand complex structureand comparison with structures from synchrotron radiation.
Acta Cryst. D 73: 702-709 (2017)
DOI: 10.1107/S2059798317008919. PubMed ID: 28777085

166) Andersson R, Safari C, Dods R, Nango E, Tanaka R, Yamashita A, Nakane T, Tono K, Joti Y, Bath P, Dunevall E, Bosman R, Nureki O, Iwata S, Neutze R and Branden G.
Serial femtosecond crystallography structure of cytochrome c oxidase at room temperature.
Sci Rep.7(1):4518 (2017)
DOI: 10.1038/s41598-017-04817-z. PubMed ID: 28674417

165) Nagarathinam K, Jaenecke F, Nakada-Nakura Y, Hotta Y, Liu K, Iwata S, Stubbs MT, Nomura N and Tanabe M.
The multidrug-resistance transporter MdfA from Escherichia coli:crystallization and X-ray diffraction analysis
Acta Cryst. F 73: 423-430 (2017)
DOI:10.1107/S2053230X17008500. PubMed ID: 28695852

164) Yasuda S, Kajiwara Y, Toyoda Y, Morimoto K, Suno R, Iwata S, Kobayashi T, Murata T and Kinoshita M.
Hot-Spot Residues to be Mutated Common in G Protein-Coupled Receptors of Class A: Identification of Thermostabilizing Mutations Followed by Determination of Three-Dimensional Structures for Two Example Receptors.
J Phys Chem B. 121(26); 6341- 6350 (2017)
DOI:10.1021/acs.jpcb.7b02997. PubMed ID: 28644022
163) Fujimori T, Suno R, Iemura SI, Natsume T, Wada I and Hosokawa N.
Endoplasmic reticulum proteins SDF2 and SDF2L1 act as components of the BiP chaperone cycle to prevent protein aggregation.
Genes Cells.22; 684-698 (2017)
DOI:10.1111/gtc.12506. PubMed ID: 28597544
162) Takeda H, Zhou W, Kido K, Suno R, Iwasaki T, Kobayashi T and Sawasaki T.
CP5 system, for simple and highly efficient protein purification with a C-terminal designed mini tag.
PLoS One.12(5): e0178246. (2017)
DOI:10.1371/journal.pone.0178246. PubMed ID: 28542437
161) Sugahara M, Nakane T, Masuda T, Suzuki M, Inoue S, Song C, Tanaka R, Nakatsu T, Mizohata E, Yumoto F, Tono K, Joti Y, Kameshima T, Hatsui T, Yabashi M, Nureki O, Numata K, Nango E and Iwata S.
Hydroxyethyl cellulose matrix applied to serial crystallography.
Sci Rep. 7: 45604 (2017)
DOI:10.1038/s41598-017-00761-0. PubMed ID: 28386083
160) Masuda T, Suzuki M, Inoue S, Song C, Nakane T, Nango E, Tanaka R, Tono K, Joti Y, Kameshima T, Hatsui T, Yabashi M, Mikami B, Nureki O, Numata K, Iwata S and Sugahara M.
Atomic resolution structure of serine protease proteinase K at ambient temperature.
Sci Rep. 7(1); 703 (2017)
DOI:10.1038/srep45604. PubMed ID: 28361898
159) Suga M, Akita F, Sugahara M, Kubo M, Nakajima Y, Nakane T, Yamashita K, Umena Y, Nakabayashi M, Yamane T, Nakano T, Suzuki M, Masuda T, Inoue S, Kimura T, Nomura T, Yonekura S, Yu LJ, Sakamoto T, Motomura T, Chen JH, Kato Y, Noguchi T, Tono K, Joti Y, Kameshima T, Hatsui T, Nango E, Tanaka R, Naitow H, Matsuura Y, Yamashita A, Yamamoto M, Nureki O, Yabashi M, Ishikawa T, Iwata S and Shen JR.
Light-induced structural changes and the site of O=O bond formation in PSII caught by XFEL.
Nature 543;131-135 (2017)
DOI:10.1038/nature21400. PubMed ID: 28219079


158) Nango E, Royant A, Kubo M, Nakane T, Wickstrand C, Kimura T, Tanaka T, Tono K, Song C, Tanaka R, Arima T, Yamashita A, Kobayashi J, Hosaka T, Mizohata E, Nogly P, Sugahara M, Nam D, Nomura T, Shimamura T, Im D, Fujiwara T, Yamanaka Y, Jeon B, Nishizawa T, Oda K, Fukuda M, Andersson R, Bath P, Dods R, Davidsson J, Matsuoka S, Kawatake S, Murata M, Nureki O, Owada S, Kameshima T, Hatsui T, Joti Y, Schertler G, Yabashi M, Bondar AN, Standfuss J, Neutze R, and Iwata S.
A three-dimensional movie of structural changes in bacteriorhodopsin.
Science. 354(6319);1552-1557(2016)
DOI:10.1126/science.aah3497. PubMed ID: 28008064
157) Kasuya G, Hiraizumi M, Maturana AD, Kumazaki K, Fujiwara Y, Liu K, Nakada-Nakura Y, Iwata S, Tsukada K, Komori T, Uemura S, Goto Y, Nakane T, Takemoto M, Kato HE, Yamashita K, Wada M, Ito K, Ishitani R, Hattori M, and Nureki O.
Crystal structures of the TRIC trimeric intracellular cation channel orthologues
Cell Res. 26(12); 1288-1301(2016)
DOI:10.1038/cr.2016.140. PubMed ID: 27909292
156) Nakane T, Hanashima S, Suzuki M, Saiki H, Hayashi T, Kakinouchi K, Sugiyama S, Kawatake S, Matsuoka S, Matsumori N, Nango E, Kobayashi J, Shimamura T, Kimura K, Mori C, Kunishima N, Sugahara M, Takakyu Y, Inoue S, Masuda T, Hosaka T, Tono K, Joti Y, Kameshima T, Hatsui T, Yabashi M, Inoue T, Nureki O , Iwata S, Murata M, and Mizohata E.
Membrane protein structure determination by SAD, SIR, or SIRAS phasing in serial femtosecond crystallography using an iododetergent
PNAS 113(46); 13039-13044 (2016)
DOI:10.1073/pnas.1602531113 PubMed ID: 27799539
155) Suno, R., Asada, H. and Kobayashi,T.
Towards the Crystal Structure Determination of Muscarinic Acetylcholine Receptors.
Springer 107: 1-13 (2016)
154) Horita, S., Nomura, Y., Sato, Y., Shimamura, T., Iwata, S. and Nomura, N.
High-resolution crystal structure of the therapeutic antibody pembrolizumab bound to the human PD-1
Scientific Reports(13) 6: 35297 (2016)
DOI:10.1038/srep35297 PubMed ID: 27734966
153) Kabe Y, Yamamoto T, Kajimura M, Sugiura Y, Koike I, Ohmura M, Nakamura T, Tokumoto Y, Tsugawa H, Handa H, Kobayashi T and Suematsu M.
Cystathionine β-synthase and PGRMC1 as CO sensors.
Free Radic Biol Med. 99; 333-344 (2016)
DOI:10.1016/j.freeradbiomed.2016.08.025 PubMed ID: 27565814
152) Nomura Y, Sato Y, Suno R, Horita S, Iwata S, Nomura N.
The intervening removable affinity tag (iRAT) production system facilitates Fv antibody fragment-mediated crystallography.
Protein Science 25 (12); 2268-2276 (2016)
DOI:10.1002/pro.3035 PubMed ID: 27595817
151) Nogly P, Panneels V, Nelson G, Gati C, Kimura T, Milne C, Milathianaki D, Kubo M, Wu W, Conrad C, Coe J, Bean R, Zhao Y, Bath P, Dods R, Harimoorthy R, Beyerlein KR, Rheinberger J, James D, DePonte D, Li C, Sala L, Williams GJ, Hunter MS, Koglin JE, Berntsen P,Nango E, Iwata S, Chapman HN, Fromme P, Frank M, Abela R, Boutet S, Barty A, White TA, Weierstall U, Spence J, Neutze R,Schertler G,and Standfuss J.
Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
Nat. Commun.7, 12314 (2016)
DOI:10.1038/ncomms12314 PubMed ID: 27545823
150) Nakane T, Joti Y, Tono K, Yabashi M, Nango E, Iwata S, Ishitania R. and Nureki O.
Data processing pipeline for serial femtosecond crystallography at SACLA
J. Appl. Cryst. 49(3); 1035-1041 (2016)
DOI:10.1107/S1600576716005720 PubMed ID: 27275146
149) Alguel Y, Amillis S, Leung J, Lambrinidis G, Capaldi S, Scull NJ, Craven G, Iwata S, Armstrong A, Mikros E, Diallinas G, Cameron AD and Byrne B.
Structure of eukaryotic purine/H(+) symporter UapA suggests a role for homodimerization in transport activity.
Nat Commun. 7; 11336 (2016)
DOI:10.1038/ncomms11336. PubMed ID: 27088252
148) Sugahara M, Song C, Suzuki M, Masuda T, Inoue S, Nakane T, Yumoto F, Nango E, Tanaka R, Tono K, Joti Y, Kameshima T, Hatsui T, Yabashi M, Nureki O, Numata K and Iwata S.
Oil-free hyaluronic acid matrix for serial femtosecond crystallography
Scientific Reports 6; 24484 (2016)
DOI:10.1038/srep24484 PubMed ID: 27087008
147) Reithmeier RA, Casey JR, Kalli A, Sansom MS, Alguel Y and Iwata S.
Band 3, the Human Red Cell Chloride/Bicarbonate Anion Exchanger (AE1, SLC4A1), in a Structural Context
Biochim Biophys Acta.1858(7); 1507-1532 (2016)
DOI:10.1016/j.bbamem.2016.03.030. PubMed ID: 27058983
146) Mafune F, Miyajima K, Tono K, Takeda Y, Kohno J, Miyauchi N, Kobayashi J, Joti Y, Nango E, Iwata S and Yabashi Y.
Microcrystal delivery by pulsed liquid droplet for serial femtosecond crystallography
Acta Cryst. D72; 520-523 (2016)
DOI:10.1107/S2059798316001480 PubMed ID: 27050131
145) Kabe Y, Nakane T, Koike I, Yamamoto T, Sugiura Y, Harada E, Sugase K, Shimamura T, Ohmura M, Muraoka K, Yamamoto A, Uchida T, Iwata S, Yamaguchi Y, Krayukhina E, Noda M, Handa H, Ishimori K, Uchiyama S, Kobayashi T and Suematsu M.
Haem-dependent dimerization of PGRMC1/Sigma-2 receptor facilitates cancer proliferation and chemoresistance
Nat Commun. 7(11030)
DOI:10.1038/ncomms11030 PubMed ID: 26988023
144) Fukuda Y, Tse KM, Nakane T, Nakatsu T, Suzuki M, Sugahara M,Inoue S, Masuda T, Yumoto F, Matsugaki N, Nango E, Tono K, Joti Y, Kameshima T, Song C, Hatsui T, Yabashi M, Nureki O, Murphy ME, Inoue T, Iwata S, and Mizohata E.
Redox-coupled proton transfer mechanism in nitrite reductase revealed by femtosecond crystallography
PNAS 113(11); 2928-2933 (2016)
DOI:10.1073/pnas.1517770113 PubMed ID: 26929369
143) Fukuda, Y., Tse, KM., Suzuki, M., Diederichs, K., Hirata, T., Nakane, T., Sugahara, M., Nango, E., Tono, K., Joti, Y., Kameshima, T., Song, C., Hatsui, T., Yabashi, M., Nureki, O., Matsumura, H., Inoue, T., Iwata, S. and Mizohata, E.
Redox-coupled structural changes in nitrite reductase revealed by serial femtosecond and microfocus crystallography
J. Biochem. 159(5); 527-538 (2016)
DOI:10.1093/jb/mvv133 PubMed ID: 26769972


142) Nakane,T., Song, C., Suzuki, M., Nango, E., Kobayashi, J., Masuda, T., Inoue, S., Mizohata, E., Nakatsu, T., Tanaka, T., Tanaka, R., Shimamura, T., Tono, K., Joti, Y., Kameshima, T., Hatsui, T., Yabashi, M., Nureki, O., Iwata, S. and Sugahara, M.
Native sulfur/chlorine SAD phasing for serial femtosecond crystallography
Acta Cryst. D71(Pt12); 2519-2525 (2015)
DOI:10.1107/S139900471501857X PubMed ID: 26627659
141) Arakawa T, Kobayashi-Yurugi T, Alguel Y, Iwanari H, Hatae H, Iwata M, Abe Y, Hino T, Ikeda-Suno C, Kuma H, Kang D, Murata T, Hamakubo T, Cameron AD, Kobayashi T, Hamasaki N, Iwata S.
Crystal structure of the anion exchanger domain of human erythrocyte band 3.
Science 350(6261): 680-684 (2015)
DOI:10.1126/science.aaa4335. PubMed ID: 26542571
140) Nomura, N., Verdon, G,. Kang, HJ., Shimamura, T., Nomura, Y., Sonoda, Y., Hussien, SA., Qureshi, AA., Coincon, M., Sato, Y., Abe, H., Nakada-Nakura, Y., Hino, T., Arakawa, T., Kusano-Arai, O., Iwanari, H., Murata, T., Kobayashi, T., Hamakubo, T., Kasahara, M., Iwata, S. and Drew, D.
Structure and mechanism of the mammalian fructose transporter GLUT5.
Nature 526; 397-401(2015)
DOI:10.1038/nature14909. PubMed ID: 26416735
139) Yamashita, K., Pan, D., Okuda, T., Sugahara, M., Kodan, A., Yamaguchi, T., Murai, T., Gomi, K., Kajiyama, N., Mizohata, E., Suzuki, M., Nango, E., Tono, K., Joti, Y., Kameshima, T., Park, J., Song, C., Hatsui, T., Yabashi, M., Iwata, S., Kato, H., Ago, H., Yamamoto, M. and Nakatsu, T.
An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography
Sci Rep.5 :14017 (2015)
DOI:10.1038/srep14017 PubMed ID: 26360462
138) Axford, D., Foadi, J., Hu, NJ., Choudhury, HG., Iwata, S., Beis, K., Evans, G. and Alguel, Y.
Structure determination of an integral membrane protein at room temperature from crystals in situ.
Acta Crystallogr D Biol Crystallogr. 71(Pt 6); 1228-1237 (2015)
DOI:10.1107/S139900471500423X. PubMed ID: 26057664
137) Tono K, Nango E, Sugahara M, Song C, Park J, Tanaka T, Tanaka R, Joti Y, Kameshima T, Ono S, Hatsui T, Mizohata E, Suzuki M, Shimamura T, Tanaka Y, Iwata S, Yabashi M.
Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): application to serial protein crystallography using an X-ray free-electron laser.
J Synchrotron Radiat. 1; 22(Pt 3); 532-537 (2015)
DOI:10.1107/S1600577515004464 PubMed ID: 25931065
136) Tanabe, H., Fujii, Y., Okada-Iwabu, M., Iwabu, M., Nakamura, Y., Hosaka, T., Motoyama, K., Ikeda, M., Wakiyama, M., Terada, T., Ohsawa, N., Hato, M., Ogasawara, S., Hino, T., Murata, T., Iwata, S., Hirata, K., Kawano, Y., Yamamoto, M., Kimura-Someya, T., Shirouzu, M., Yamauchi, T., Kadowaki, T. and Yokoyama, S.
Crystal structures of the human adiponectin receptors
Nature. 520; 312-316 (2015)
DOI:10.1038/nature14301 PubMed ID: 25855295
135) Shiroishi, M., Kobayashi,T.
Screening of Stable G-Protein-Coupled Receptor Variants in Saccharomyces cerevisiae.
Methods Mol Biol. 1261;159-170 (2015)
DOI:10.1007/978-1-4939-2230-7_9 PubMed ID: 25502199
134) Tanabe, H., Motoyama, K., Ikeda, M., Wakiyama, M., Terada, T., Ohsawa, N., Hosaka, T., Hato, M., Fujii, Y., Nakamura, Y., Ogasawara, S., Hino, T., Murata, T., Iwata, S., Okada-Iwabu, M., Iwabu, M., Hirata, K., Kawano, Y., Yamamoto, M., Kimura-Someya, T., Shirouzu, M., Yamauchi, T., Kadowaki, T. and Yokoyama, S.
Expression, purification, crystallization, and preliminary X-ray crystallographic studies of the human adiponectin receptors, AdipoR1 and AdipoR2.
J Struct Funct Genomics. 16(1); 11-23 (2015)
DOI:10.1007/s10969-014-9192-z PubMed ID: 25575462
133) Hu, N-Jen., Rada, H., Rahman, N., Nettleship, J.E., Bird, L., Iwata, S., Drew, D., Cameron, A.D., and Owens, R.J.
GFP-Based Expression Screening of Membrane Proteins in Insect Cells Using the Baculovirus System.
Springer. 1261 (3) 197-209 (2015)
DOI:10.1007/978-1-4939-2230-7_11 PubMed ID: 25502201
132) Sugahara,M., Mizohata,E., Nango,E., Suzuki,M., Tanaka,T., Masuda,T., Tanaka,T., Shimamura,T., Tanaka,Y., Suno,C., Ihara,K., Pan,D., Kakinouchi,K., Sugiyama,S., Murata,M., Inoue,T., Tono,K., Song,C., Park,J., Kameshima,T., Hatsui,T., Joti,Y., Yabashi,M., Iwata,S.
Grease matrix as a versatile carrier of proteins for serial crystallography
Nature Methods 12, 61-63 (2015)
DOI:10.1038/nmeth.3172 PubMed ID: 25384243


131)  Suharni, Nomura, Y., Arakawa, T., Hino, T., Abe, H., Nakada-Nakura, Y., Sato, Y., Iwanari, H., Shiroishi, M., Asada, H., Shimamura, T., Murata, T., Kobayashi, T., Hamakubo, T., Iwata, S. and Nomura, N.
Proteoliposome-based selection of a recombinant antibody fragment against the human M2 muscarinic acetylcholine receptor.
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 33(6); 378-385(2014)
DOI:10.1089/mab.2014.0041 PubMed ID: 25545206
130) Lee, C., Yashiro, S., Dotson, DL., Uzdavinys, P., Iwata, S., Sansom, MS., von Ballmoos, C., Beckstein, O., Drew, D. and Cameron, A.D.
Crystal structure of the sodium-proton antiporter NhaA dimer and new mechanistic insights.
J.Gen.Physiol. 144 (6); 529-544 (2014)
DOI:10.1085/jgp.201411219 PubMed ID: 25422503
129) Adachi, M., Kawasaki, A., Nojima, H., Nishida, E., Tsukita, S.
Involvement of IQGAP family proteins in the regulation of mammalian cell cytokinesis
Genes to Cells 19(11); 803-820(2014)
DOI:10.1111/gtc.12179 PubMed ID: 25229330
128) Koike-Takeshita, A., Arakawa, T., Taguchi, H., Shimamura,T.
Crystal structure of a symmetric football-shaped GroEL:GroES2complex determined at 3.8Å reveals rearrangement between two GroEL rings
J Mol Biol.426(21); 3634-3641(2014)
DOI:10.1016/j.jmb.2014.08.017. PubMed ID: 25174333
127)  Simmons, KJ., Jackson, SM., Brueckner, F., Patching, SG., Beckstein, O., Ivanova, E., Geng, T., Weyand, S., Drew, D., Lanigan, J., Sharples, DJ., Sansom, MS., Iwata, S., Fishwick, CW., Johnson, AP., Cameron, AD., Henderson, PJ.
Molecular mechanism of ligand recognition by membrane transport protein, Mhp1
EMBO. 33(12); 1285-1415 (2014)
DOI:10.15252/embj.201387557. PubMed ID: 24952894
126)  Choudhury, HG., Tong, Z., Mathavan, I., Li, Y., Iwata, S., Zirah, S., Rebuffat, S., van Veen, HW., Beis, K.
Structure of an antibacterial peptide ATP-binding cassette transporter in a novel outward occluded state
PNAS. 111(25); 9145-9150 (2014)
DOI:10.1073/pnas.1320506111. PubMed ID: 24920594
125)  Gallat, FX., Matsugaki, N., Coussens, NP., Yagi, KJ., Boudes, M., Higashi, T., Tsuji, D., Tatano, Y., Suzuki, M., Mizohata, E., Tono, K., Joti, Y., Kameshima, T., Park, J., Song, C., Hatsui, T., Yabashi, M., Nango, E., Itoh, K., Coulibaly, F., Tobe, S., Ramaswamy, S., Stay, B., Iwata, S., Chavas, LM.
In vivo crystallography at X-ray free-electron lasers: the next generation of structural biology?
Phil. Trans. R. Soc. B. 369(1647); 20130497 (2014)
DOI:10.1098/rstb.2013.0497. PubMed ID: 24914164


124) Alam, J., Yamato, I., Arai, S., Saijo, S., Mizutani, K., Ishizuka-Katsura, Y., Ohsawa, N., Terada, T., Shirouzu, M., Yokoyama, S., Iwata, S., Kakinuma, Y., Murata, T.
Mutant LV(476-7)AA of A-subunit of Enterococcus hirae V1-ATPase: High affinity of A3B3 complex to DF axis and low ATPase activity.
Springerplus. 2; 689 (2013)
DOI:10.1186/2193-1801-2-689 PubMed ID: 24404436
123) Manolaridis, I., Kulkarni, K., Dodd, RD., Ogasawara, S., Zhang, Z., Bineva, G., O’Reilly, N., Hanrahan, SJ., Thompson, AJ., Cronin, N., S, Iwata., Barford, D.
Mechanism of farnesylated CAAX protein processing by the intramembrane protease Rce1.
Nature. 504(7479):301-305(2013)
DOI:10.1038/nature12754 PubMed ID: 24291792
Press Release:がんを引き起こす膜たんぱく質の立体構造と働きを解明 ~がんを抑制する薬剤の設計へ~
122) Lee C, Kang HJ, von Ballmoos C, Newstead S, Uzdavinys P, Dotson DL, Iwata S, Beckstein O, Cameron AD, Drew D.
A two-domain elevator mechanism for sodium/proton antiport.
Nature. 501(7468):573-7.(2013)
DOI:10.1038/nature12484 PubMed ID:23995679
121) Alam MJ, Arai S, Saijo S, Suzuki K, Mizutani K, Ishizuka-Katsura Y, Ohsawa N, Terada T, Shirouzu M, Yokoyama S, Iwata S, Kakinuma Y, Yamato I, Murata T.
Loose Binding of the DF Axis with the A3B3 Complex Stimulates the Initial Activity of Enterococcus hirae V1-ATPase.
PLoS One. 8(9):e74291.(2013)
DOI:10.1371/journal.pone.0074291 PubMed ID:24058539
120) Foadi J, Aller P, Alguel Y, Cameron A, Axford D, Owen RL, Armour W, Waterman DG, Iwata S, Evans G.
Clustering procedures for the optimal selection of data sets from multiple crystals in macromolecular crystallography.
Acta Crystallogr D Biol Crystallogr. 69(8); 1617-1632 (2013)
DOI:10.1107/S0907444913012274 PubMed ID:23897484
119) Murai Y, Masuda K, Ogasawara Y, Wang L, Hashidoko Y, Hatanaka Y, Iwata S, Kobayashi T, Hashimoto M.
Synthesis of Photoreactive 2-Phenethylamine Derivatives - Synthesis of Adenosine Derivatives Enabling Functional Analysis of Adenosine Receptors by Photoaffinity Labeling.
European J Org Chem. 2013(12); 2428-2433 (2013)
118) Rahman S, Yamato I, Saijo S, Mizutani K, Katsura-Ishizuka Y, Ohsawa N, Terada T, Shirouzu M, Yokoyama S, Iwata S, Murata T.
Biochemical and biophysical properties of interactions between subunits of the peripheral stalk region of human V-ATPase.
PloS One 8(2); 1-13 (2013)
DOI:10.1371/journal.pone.0055704 PubMed ID:23409023
117) Hino T, Iwata S, Murata T.
Generation of functional antibodies for mammalian membrane protein crystallography.
Curr Opin Struct Biol. 2013 May 8. pii: S0959-440X(13)00074-2.
DOI:10.1016/j.sbi.2013.04.007. PubMed ID:23664057
116) Arai S, Saijo S, Suzuki K, Mizutani K, Kakinuma Y, Ishizuka-Katsura Y, Ohsawa N, Terada T, Shirouzu M, Yokoyama S, Iwata S, Yamato I, Murata T.
Rotation mechanism of Enterococcus hirae V1-ATPase based on asymmetric crystal structures.
Nature. 2013 Jan 31;493(7434):703-7.
DOI:10.1038/nature11778 PubMed ID:23334411
Related PDB Entries:3VR6, 3VR5, 3VR4, 3VR3, 3VR2, 3VR1

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115) Iwata M, Lee Y, Yamashita T, Yagi T, Iwata S, Cameron AD, Maher MJ.
The structure of the yeast NADH dehydrogenase (Ndi1) reveals overlapping binding sites for water- and lipid-soluble substrates.
Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15247-52.
DOI:10.1073/pnas.1210059109 PubMed ID:22949654
Related PDB Entries:4GAV, 4GAP, 4G9K
114) Suno R, Shimoyama M, Abe A, Shimamura T, Shimodate N, Watanabe YH, Akiyama Y, Yoshida M.
Conformational transition of the lid helix covering the protease active site is essential for the ATP-dependent protease activity of FtsH.
FEBS Lett. 2012 Sep 21;586(19):3117-21.
DOI:10.1016/j.febslet.2012.07.069 PubMed ID:23166924
113) Solcan N, Kwok J, Fowler PW, Cameron AD, Drew D, Iwata S, Newstead S.
Alternating access mechanism in the POT family of oligopeptide transporters.
EMBO J. 2012 Aug 15;31(16):3411-21.
DOI:10.1038/emboj.2012.157 PubMed ID:22659829
Related PDB Entries:4APS
112) Sasaki H, Kubota K, Lee WC, Ohtsuka J, Kojima M, Iwata S, Nakagawa A, Takahashi K, Tanokura M.
The crystal structure of an intermediate dimer of aspergilloglutamic peptidase that mimics the enzyme-activation product complex produced upon autoproteolysis.
J Biochem. 2012 Jul;152(1):45-52.
DOI:10.1093/jb/mvs050 PubMed ID:22569035
Related PDB Entries:3TRS
111) Shiroishi M, Tsujimoto H, Makyio H, Asada H, Yurugi-Kobayashi T, Shimamura T, Murata T, Nomura N, Haga T, Iwata S, Kobayashi T.
Platform for the rapid construction and evaluation of GPCRs for crystallography in Saccharomyces cerevisiae.
Microb Cell Fact. 2012 Jun 13;11:78.
DOI:10.1186/1475-2859-11-78 PubMed ID:22694812
110) Hino T, Arakawa T, Iwanari H, Yurugi-Kobayashi T, Ikeda-Suno C, Nakada-Nakura Y, Kusano-Arai O, Weyand S, Shimamura T, Nomura N, Cameron AD, Kobayashi T, Hamakubo T, Iwata S, Murata T.
G-protein-coupled receptor inactivation by an allosteric inverse-agonist antibody.
Nature. 2012 Jan 29;482(7384):237-40.
DOI:10.1038/nature10750 PubMed ID:22286059
Related PDB Entries:3VGA, 3VG9
Press Release:抗体を用いて創薬標的膜たんぱく質の結晶構造を得ることに成功 -全く新しい阻害機構を持った薬剤の設計が可能に-
109) Haga K, Kruse AC, Asada H, Yurugi-Kobayashi T, Shiroishi M, Zhang C, Weis WI, Okada T, Kobilka BK, Haga T, Kobayashi T.
Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist.
Nature. 2012 Jan 25;482(7386):547-51.
DOI:10.1038/nature10753 PubMed ID:22278061
Related PDB Entries:3UON
Press Release:認知症や心機能の抑制に関係する受容体の立体構造を世界で初めて解明-より効果的で副作用のない治療薬の探索・設計が可能に-

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108) Mizutani K, Yoshioka S, Mizutani Y, Iwata S, Mikami B.
High-throughput construction of expression system using yeast Pichia pastoris, and its application to membrane proteins.
Protein Expr Purif. 2011 May;77(1):1-8.
DOI:10.1016/j.pep.2010.12.009 PubMed ID:21172439
107) Ito K, Ito S, Shimamura T, Weyand S, Kawarasaki Y, Misaka T, Abe K, Kobayashi T, Cameron AD, Iwata S.
Crystal structure of glucansucrase from the dental caries pathogen Streptococcus mutans.
J Mol Biol. 2011 Apr 29;408(2):177-86.
DOI:10.1016/j.jmb.2011.02.028 PubMed ID:21354427
Related PDB Entries:3AIE, 3AIC, 3AIB
Press Release:虫歯の病原因子である酵素の立体構造を世界で初めて解明 ~虫歯の予防物質を探索するための手がかりを得る~
106) Rahman S, Ishizuka-Katsura Y, Arai S, Saijo S, Yamato I, Toyama M, Ohsawa N, Inoue M, Honda K, Terada T, Shirouzu M, Yokoyama S, Iwata S, Murata T.
Expression, purification and characterization of isoforms of peripheral stalk subunits of human V-ATPase.
Protein Expr Purif. 2011 Aug;78(2):181-8.
DOI:10.1016/j.pep.2011.02.012 PubMed ID:21356312
105) de Graaf C, Kooistra AJ, Vischer HF, Katritch V, Kuijer M, Shiroishi M, Iwata S, Shimamura T, Stevens RC, de Esch IJ, Leurs R.
Crystal structure-based virtual screening for fragment-like ligands of the human histamine H(1) receptor.
J Med Chem. 2011 Dec 8;54(23):8195-206.
DOI:10.1021/jm2011589 PubMed ID:22007643
104) Shiroishi M, Kobayashi T, Ogasawara S, Tsujimoto H, Ikeda-Suno C, Iwata S, Shimamura T.
Production of the stable human histamine H1 receptor in Pichia pastoris for structural determination.
Methods. 2011 Dec;55(4):281-6.
DOI:10.1016/j.ymeth.2011.08.015 PubMed ID:21903167
103) Choudhury HG, Cameron AD, Iwata S, Beis K.
Structure and mechanism of the chalcogen-detoxifying protein TehB from Escherichia coli
Biochem J. 2011 Apr 1;435(1):85-91.
DOI:10.1042/BJ20102014 PubMed ID:21244361
Related PDB Entries:2XVM, 2XVA
102) Asada H, Uemura T, Yurugi-Kobayashi T, Shiroishi M, Shimamura T, Tsujimoto H, Ito K, Sugawara T, Nakane T, Nomura N, Murata T, Haga T, Iwata S, Kobayashi T.
Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis.
Microb Cell Fact. 2011 Apr 22;10:24.
DOI:10.1186/1475-2859-10-24 PubMed ID:21513509
101) Tokuda N, Igarashi K, Shimamura T, Yurugi-Kobayashi T, Shiroishi M, Ito K, Sugawara T, Asada H, Murata T, Nomura N, Iwata S, Kobayashi T.
Cloning, expression and purification of the anion exchanger 1 homologue from the basidiomycete Phanerochaete chrysosporium.
Protein Expr Purif. 2011 Sep;79(1):81-7.
DOI:10.1016/j.pep.2011.04.006 PubMed ID:21515379
100) Saijo S, Arai S, Hossain KM, Yamato I, Suzuki K, Kakinuma Y, Ishizuka-Katsura Y, Ohsawa N, Terada T, Shirouzu M, Yokoyama S, Iwata S, Murata T.
Crystal structure of the central axis DF complex of the prokaryotic V-ATPase.
Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):19955-60.
DOI:10.1073/pnas.1108810108 PubMed ID:22114184
Related PDB Entries:3AON
Press Release:タンパク質ナノモーターの回転軸の詳細構造を解明-骨粗鬆症に関与する酵素の活性調節領域を同定-
99) Hu NJ, Iwata S, Cameron AD, Drew D.
Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT.
Nature. 2011 Oct 5;478(7369):408-11.
DOI:10.1038/nature10450 PubMed ID:21976025
Related PDB Entries:3ZUY, 3ZUX
Press Release:コレステロールを調節するたんぱく質の立体構造を世界で初めて解明-副作用の少ない、高脂血症や動脈硬化症の治療薬の開発へ-
98) Mizutani K, Yamamoto M, Suzuki K, Yamato I, Kakinuma Y, Shirouzu M, Walker JE, Yokoyama S, Iwata S, Murata T.
Structure of the rotor ring modified with N,N'-dicyclohexylcarbodiimide of the Na+-transporting vacuolar ATPase.
Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):13474-9.
DOI:10.1073/pnas.1103287108 PubMed ID:21813759
Related PDB Entries:3AOU, 2DB4,
Press Release:V型ATPaseの阻害機構の解明に成功-骨粗鬆症やがんの新薬開発に繋がる成果-
97) Shimamura T, Shiroishi M, Weyand S, Tsujimoto H, Winter G, Katritch V, Abagyan R, Cherezov V, Liu W, Han GW, Kobayashi T, Stevens RC, Iwata S.
Structure of the human histamine H1 receptor complex with doxepin.
Nature. 2011 Jun 22;475(7354):65-70.
DOI:10.1038/nature10236 PubMed ID:21697825
Related PDB Entries:3RZE
Press Release:花粉症・アレルギーの発症因子の立体構造を世界で初めて解明-副作用を抑えた治療薬の探索・設計が可能に-
96) Newstead S, Drew D, Cameron AD, Postis VL, Xia X, Fowler PW, Ingram JC, Carpenter EP, Sansom MS, McPherson MJ, Baldwin SA, Iwata S.
Crystal structure of a prokaryotic homologue of the mammalian oligopeptide-proton symporters, PepT1 and PepT2.
EMBO J. 2011 Jan 19;30(2):417-26.
DOI:10.1038/emboj.2010.309 PubMed ID:21131908
Related PDB Entries:2XUT
95) Bill RM, Henderson PJ, Iwata S, Kunji ER, Michel H, Neutze R, Newstead S, Poolman B, Tate CG, Vogel H.
Overcoming barriers to membrane protein structure determination.
Nat Biotechnol. 2011 Apr;29(4):335-40.
DOI:10.1038/nbt.1833 PubMed ID:21478852
94) Ruprecht J, Iwata S, Rothery RA, Weiner JH, Maklashina E, Cecchini G.
Perturbation of the quinone-binding site of complex II alters the electronic properties of the proximal [3Fe-4S] iron-sulfur cluster.
J Biol Chem. 2011 Apr 8;286(14):12756-65.
DOI:10.1074/jbc.M110.209874 PubMed ID:21310949
Related PDB Entries:2WP9
93) Sonoda Y, Newstead S, Hu NJ, Alguel Y, Nji E, Beis K, Yashiro S, Lee C, Leung J, Cameron AD, Byrne B, Iwata S, Drew D.
Benchmarking membrane protein detergent stability for improving throughput of high-resolution X-ray structures.
Structure. 2011 Jan 12;19(1):17-25.
DOI:10.1016/j.str.2010.12.001 PubMed ID:21220112
92) Weyand S, Shimamura T, Beckstein O, Sansom MS, Iwata S, Henderson PJ, Cameron AD.
The alternating access mechanism of transport as observed in the sodium-hydantoin transporter Mhp1.
J Synchrotron Radiat. 2011 Jan;18(1):20-3.
DOI:10.1107/S0909049510032449 PubMed ID:21169684
91) Ito K, Sugawara T, Koizumi A, Nakajima K, Shimizu-Ibuka A, Shiroishi M, Asada H, Yurugi-Kobayashi T, Shimamura T, Asakura T, Misaka T, Iwata S, Kobayashi T, Abe K.
Cysteine-to-serine shuffling using a Saccharomyces cerevisiae expression system improves protein secretion: case of a nonglycosylated mutant of miraculin, a taste-modifying protein.
Biotechnol Lett. 2011 Jan;33(1):103-7.
DOI:10.1007/s10529-010-0399-1 PubMed ID:20936326

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90) Hino T, Matsumoto Y, Nagano S, Sugimoto H, Fukumori Y, Murata T, Iwata S, Shiro Y.
Structural basis of biological N2O generation by bacterial nitric oxide reductase.
Science. 2010 Dec 17;330(6011):1666-70.
DOI:10.1126/science.1195591 PubMed ID:21109633
Related PDB Entries:3O0R
Press Release:温室効果ガス「亜酸化窒素」を発生させる酵素の立体構造を世界で初めて解明 -嫌気呼吸から酸素呼吸へと呼吸酵素が進化した手がかりを得る-
89) Suzuki N, Hiraki M, Yamada Y, Matsugaki N, Igarashi N, Kato R, Dikic I, Drew D, Iwata S, Wakatsuki S, Kawasaki M.
Crystallization of small proteins assisted by green fluorescent protein.
Acta Crystallogr D Biol Crystallogr. 2010 Oct;66(Pt 10):1059-66.
DOI:10.1107/S0907444910032944 PubMed ID:20944239
Related PDB Entries:3AI5, 3AI4
88) Ito K, Ito S, Shimamura T, Kawarasaki Y, Abe K, Misaka T, Kobayashi T, Iwata S.
Crystallization and preliminary X-ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Sep 1;66(Pt 9):1086-8.
DOI:10.1107/S1744309110029714 PubMed ID:20823533
87) Ito K, Sugawara T, Koizumi A, Nakajima K, Shimizu-Ibuka A, Shiroishi M, Asada H, Yurugi-Kobayashi T, Shimamura T, Asakura T, Masuda K, Ishiguro M, Misaka T, Iwata S, Kobayashi T, Abe K.
Bulky high-mannose-type N-glycan blocks the taste-modifying activity of miraculin.
Biochim Biophys Acta. 2010 Sep;1800(9):986-92.
DOI:10.1016/j.bbagen.2010.06.003 PubMed ID:20542090
86) Weyand S, Iwata S.
Biochemistry. Old gate gets a new look.
(Comment on “Structure of the human BK channel Ca2+-activation apparatus at 3.0 A resolution. [Science. 2010]”)
Science. 2010 Jul 9;329(5988):151-2.
DOI:10.1126/science.1192680 PubMed ID:20616256
85) Sonoda Y, Cameron A, Newstead S, Omote H, Moriyama Y, Kasahara M, Iwata S, Drew D.
Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins.
FEBS Lett. 2010 Jun 18;584(12):2539-47.
DOI:10.1016/j.febslet.2010.04.015 PubMed ID:20394746
84) Singh S, Hedley D, Kara E, Gras A, Iwata S, Ruprecht J, Strange PG, Byrne B.
A purified C-terminally truncated human adenosine A(2A) receptor construct is functionally stable and degradation resistant.
Protein Expr Purif. 2010 Nov;74(1):80-7.
DOI:10.1016/j.pep.2010.04.018 PubMed ID:20438843
83) Beale J, Lee SY, Iwata S, Beis K.
Structure of the aliphatic sulfonate-binding protein SsuA from Escherichia coli.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Apr 1;66(Pt 4):391-6.
DOI:10.1107/S1744309110006226 PubMed ID:20383006
Related PDB Entries:2X26
82) Shimamura T, Weyand S, Beckstein O, Rutherford NG, Hadden JM, Sharples D, Sansom MS, Iwata S, Henderson PJ, Cameron AD.
Molecular basis of alternating access membrane transport by the sodium-hydantoin transporter Mhp1.
Science. 2010 Apr 23;328(5977):470-3.
DOI:10.1126/science.1186303 PubMed ID:20413494
Related PDB Entries:2X79
Press Release:細胞膜たんぱく質が物質を細胞内へ運ぶ仕組みを分子レベルで解明 (神経疾患やがんの治療に役立つ可能性)

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81) Arai S, Yamato I, Shiokawa A, Saijo S, Kakinuma Y, Ishizuka-Katsura Y, Toyama M, Terada T, Shirouzu M, Yokoyama S, Iwata S, Murata T.
Reconstitution in vitro of the catalytic portion (NtpA3-B3-D-G complex) of Enterococcus hirae V-type Na+-ATPase.
Biochem Biophys Res Commun. 2009 Dec 18;390(3):698-702.
DOI:10.1016/j.bbrc.2009.10.032 PubMed ID:19833097
80) Maher MJ, Akimoto S, Iwata M, Nagata K, Hori Y, Yoshida M, Yokoyama S, Iwata S, Yokoyama K.
Crystal structure of A3B3 complex of V-ATPase from Thermus thermophilus.
EMBO J. 2009 Dec 2;28(23):3771-9.
DOI:10.1038/emboj.2009.310 PubMed ID:19893485
Related PDB Entries:3GQB
79) Newstead S, Fowler PW, Bilton P, Carpenter EP, Sadler PJ, Campopiano DJ, Sansom MS, Iwata S.
Insights into how nucleotide-binding domains power ABC transport.
Structure. 2009 Sep 9;17(9):1213-22.
DOI:10.1016/j.str.2009.07.009 PubMed ID:19748342
Related PDB Entries:3FVQ
Press Release:
78) Ruprecht J, Yankovskaya V, Maklashina E, Iwata S, Cecchini G.
Structure of Escherichia coli succinate:quinone oxidoreductase with an occupied and empty quinone-binding site.
J Biol Chem. 2009 Oct 23;284(43):29836-46.
DOI:10.1074/jbc.M109.010058 PubMed ID:19710024
Related PDB Entries:2WDR, 2WDV, 2WDQ
77) Sugawara T, Ito K, Shiroishi M, Tokuda N, Asada H, Yurugi-Kobayashi T, Shimamura T, Misaka T, Nomura N, Murata T, Abe K, Iwata S, Kobayashi T.
Fluorescence-based optimization of human bitter taste receptor expression in Saccharomyces cerevisiae.
Biochem Biophys Res Commun. 2009 May 15;382(4):704-10.
DOI:10.1016/j.bbrc.2009.03.089 PubMed ID:19306845
76) Andrell J, Hicks MG, Palmer T, Carpenter EP, Iwata S, Maher MJ.
Crystal structure of the acid-induced arginine decarboxylase from Escherichia coli: reversible decamer assembly controls enzyme activity.
Biochemistry. 2009 May 12;48(18):3915-27.
DOI:10.1021/bi900075d PubMed ID:19298070
Related PDB Entries:2VYC
Press Release:
75) Yurugi-Kobayashi T, Asada H, Shiroishi M, Shimamura T, Funamoto S, Katsuta N, Ito K, Sugawara T, Tokuda N, Tsujimoto H, Murata T, Nomura N, Haga K, Haga T, Iwata S, Kobayashi T.
Comparison of functional non-glycosylated GPCRs expression in Pichia pastoris.
Biochem Biophys Res Commun. 2009 Mar 6;380(2):271-6.
DOI:10.1016/j.bbrc.2009.01.053 PubMed ID:19167344

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74) Shimamura T, Yajima S, Suzuki S, Rutherford NG, O'Reilly J, Henderson PJ, Iwata S.
Crystallization of the hydantoin transporter Mhp1 from Microbacterium liquefaciens.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Dec 1;64(Pt 12):1172-4.
DOI:10.1107/S1744309108036920 PubMed ID:19052379
73) Nomura N, Nomura Y, Sussman D, Klein D, Stoddard BL.
Recognition of a common rDNA target site in archaea and eukarya by analogous LAGLIDADG and His-Cys box homing endonucleases.
Nucleic Acids Res. 2008 Dec;36(22):6988-98.
DOI:10.1093/nar/gkn846 PubMed ID:18984620
Related PDB Entries:3E54
72) Drew D, Klepsch MM, Newstead S, Flaig R, De Gier JW, Iwata S, Beis K.
The structure of the efflux pump AcrB in complex with bile acid.
Mol Membr Biol. 2008 Dec;25(8):677-82.
DOI:10.1080/09687680802552257 PubMed ID:19023693
Related PDB Entries:2W1B
71) Newstead S, Hobbs J, Jordan D, Carpenter EP, Iwata S.
Insights into outer membrane protein crystallization.
Mol Membr Biol. 2008 Dec;25(8):631-8.
DOI:10.1080/09687680802526574 PubMed ID:19023694
70) Carpenter EP, Beis K, Cameron AD, Iwata S.
Overcoming the challenges of membrane protein crystallography.
Curr Opin Struct Biol. 2008 Oct;18(5):581-6.
DOI:10.1016/j.sbi.2008.07.001 PubMed ID:18674618
69) Yamamoto M, Unzai S, Saijo S, Ito K, Mizutani K, Suno-Ikeda C, Yabuki-Miyata Y, Terada T, Toyama M, Shirouzu M, Kobayashi T, Kakinuma Y, Yamato I, Yokoyama S, Iwata S, Murata T.
Interaction and stoichiometry of the peripheral stalk subunits NtpE and NtpF and the N-terminal hydrophilic domain of NtpI of Enterococcus hirae V-ATPase.
J Biol Chem. 2008 Jul 11;283(28):19422-31.
DOI:10.1074/jbc.M801772200 PubMed ID:18460472
68) Murata T, Yamato I, Kakinuma Y, Shirouzu M, Walker JE, Yokoyama S, Iwata S.
Ion binding and selectivity of the rotor ring of the Na+-transporting V-ATPase.
Proc Natl Acad Sci U S A. 2008 Jun 24;105(25):8607-12.
DOI:10.1073/pnas.0800992105. PubMed ID:18559856
Press Release:イオン輸送性ATPaseの輸送のメカニズムの一端を解明~骨粗鬆症やがん治療の理解に役立つ可能性~
67) Weyand S, Shimamura T, Yajima S, Suzuki S, Mirza O, Krusong K, Carpenter EP, Rutherford NG, Hadden JM, O'Reilly J, Ma P, Saidijam M, Patching SG, Hope RJ, Norbertczak HT, Roach PC, Iwata S, Henderson PJ, Cameron AD.
Structure and molecular mechanism of a nucleobase-cation-symport-1 family transporter.
Science. 2008 Oct 31;322(5902):709-13.
DOI:10.1126/science.1164440 PubMed ID:18927357
Related PDB Entries:2JLO, 2JLN
Press Release:細胞膜の「輸送体」が物質を輸送する巧妙な仕組みを解明
66) Jormakka M, Yokoyama K, Yano T, Tamakoshi M, Akimoto S, Shimamura T, Curmi P, Iwata S.
Molecular mechanism of energy conservation in polysulfide respiration.
Nat Struct Mol Biol. 2008 Jul;15(7):730-7.
DOI:10.1038/nsmb.1434 PubMed ID:18536726
Related PDB Entries:2VPZ, 2VPY, 2VPX, 2VPW
65) Ito K, Sugawara T, Shiroishi M, Tokuda N, Kurokawa A, Misaka T, Makyio H, Yurugi-Kobayashi T, Shimamura T, Nomura N, Murata T, Abe K, Iwata S, Kobayashi T.
Advanced method for high-throughput expression of mutated eukaryotic membrane proteins in Saccharomyces cerevisiae.
Biochem Biophys Res Commun. 2008 Jul 11;371(4):841-5.
DOI:10.1016/j.bbrc.2008.04.182 PubMed ID:18474222
64) Kouwen TR, Andrell J, Schrijver R, Dubois JY, Maher MJ, Iwata S, Carpenter EP, van Dijl JM.
Thioredoxin A active-site mutants form mixed disulfide dimers that resemble enzyme-substrate reaction intermediates.
J Mol Biol. 2008 Jun 6;379(3):520-34.
DOI:10.1016/j.jmb.2008.03.077 PubMed ID:18455736
Related PDB Entries:2VOC
63) Drew D, Newstead S, Sonoda Y, Kim H, von Heijne G, Iwata S.
GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae.
Nat Protoc. 2008;3(5):784-98.
DOI:10.1038/nprot.2008.44 PubMed ID:18451787
62) Newstead S, Ferrandon S, Iwata S.
Rationalizing alpha-helical membrane protein crystallization.
Protein Sci. 2008 Mar;17(3):466-72.
DOI:10.1110/ps.073263108 PubMed ID:18218713

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61) Tanabe M, Mirza O, Bertrand T, Atkins HS, Titball RW, Iwata S, Brown KA, Byrne B.
Structures of OppA and PstS from Yersinia pestis indicate variability of interactions with transmembrane domains.
Acta Crystallogr D Biol Crystallogr. 2007 Nov;63(Pt 11):1185-93.
PubMed ID:18007034
Related PDB Entries:2Z23, 2Z22
60) Guan L, Mirza O, Verner G, Iwata S, Kaback HR.
Structural determination of wild-type lactose permease.
Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15294-8.
PubMed ID:17881559
Related PDB Entries:2V8N
59) Newstead S, Kim H, von Heijne G, Iwata S, Drew D.
High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae.
Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):13936-41.
PubMed ID:17709746

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58) Horsefield R, Yankovskaya V, Sexton G, Whittingham W, Shiomi K, Omura S, Byrne B, Cecchini G, Iwata S.
Structural and computational analysis of the quinone-binding site of complex II (succinate-ubiquinone oxidoreductase): a mechanism of electron transfer and proton conduction during ubiquinone reduction.
J Biol Chem. 2006 Mar 17;281(11):7309-16.
PubMed ID:16407191
Related PDB Entries:2ACZ
57) Mirza O, Guan L, Verner G, Iwata S, Kaback HR.
Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY.
EMBO J. 2006 Mar 22;25(6):1177-83. Erratum in EMBO J. 2006 May 3;25(9):2038.
PubMed ID:16525509
Related PDB Entries:2CFQ, 2CFP
56) Shimizu-Ibuka A, Morita Y, Terada T, Asakura T, Nakajima K, Iwata S, Misaka T, Sorimachi H, Arai S, Abe K.
Crystal structure of neoculin: insights into its sweetness and taste-modifying activity.
J Mol Biol. 2006 May 26;359(1):148-58.
PubMed ID:16616933
Related PDB Entries:2D04

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55) Makyio H, Iino R, Ikeda C, Imamura H, Tamakoshi M, Iwata M, Stock D, Bernal RA, Carpenter EP, Yoshida M, Yokoyama K, Iwata S.
Structure of a central stalk subunit F of prokaryotic V-type ATPase/synthase from Thermus thermophilus.
EMBO J. 2005 Nov 16;24(22):3974-83.
PubMed ID:16281059
Related PDB Entries:2D00

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54) Abramson J, Iwata S, Kaback HR.
Lactose permease as a paradigm for membrane transport proteins (Review).
Mol Membr Biol. 2004 Jul-Aug;21(4):227-36.
PubMed ID:15371012
53) Iwata S, Barber J.
Structural comparison of lactose permease and the glycerol-3-phosphate antiporter: members of the major facilitator superfamily.
Curr Opin Struct Biol. 2004 Aug;14(4):413-9.
PubMed ID:15313234
52) Shimamura T, Shamotienko O, Akhtar S, Dolly JO, Iwata S.
Crystallization and preliminary X-ray analysis of pseudo-merohedrally twinned crystals of the full-length beta(2) subunit of the Kv1 K(+) channel from Rattus norvegicus.
Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):912-4.
PubMed ID15103137:
51) Horsefield R, Iwata S, Byrne B.
Complex II from a structural perspective.
Curr Protein Pept Sci. 2004 Apr;5(2):107-18.
PubMed ID:15078221
50) Shimamura T, Koike-Takeshita A, Yokoyama K, Masui R, Murai N, Yoshida M, Taguchi H, Iwata S.
Crystal structure of the native chaperonin complex from Thermus thermophilus revealed unexpected asymmetry at the cis-cavity.
Structure. 2004 Aug;12(8):1471-80.
PubMed ID:15296740
Related PDB Entries:1WE3
49) Ferreira KN, Iverson TM, Maghlaoui K, Barber J, Iwata S.
Architecture of the photosynthetic oxygen-evolving center.
Science. 2004 Mar 19;303(5665):1831-8.
PubMed ID:14764885
Related PDB Entries:1S5L
48) Jormakka M, Richardson D, Byrne B, Iwata S.
Architecture of NarGH reveals a structural classification of Mo-bisMGD enzymes.
Structure. 2004 Jan;12(1):95-104.
PubMed ID:14725769
Related PDB Entries:1R27
47) Iwata M, Imamura H, Stambouli E, Ikeda C, Tamakoshi M, Nagata K, Makyio H, Hankamer B, Barber J, Yoshida M, Yokoyama K, Iwata S.
Crystal structure of a central stalk subunit C and reversible association/dissociation of vacuole-type ATPase.
Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):59-64.
PubMed ID:14684831
Related PDB Entries:1R5Z

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46) Abramson J, Smirnova I, Kasho V, Verner G, Iwata S, Kaback HR.
The lactose permease of Escherichia coli: overall structure, the sugar-binding site and the alternating access model for transport.
FEBS Lett. 2003 Nov 27;555(1):96-101.
PubMed ID:14630326
45) Shimamura T, Koike-Takeshita A, Yokoyama K, Yoshida M, Taguchi H, Iwata S.
Crystallization of the chaperonin GroEL-GroES complex from Thermus thermophilus HB8.
Acta Crystallogr D Biol Crystallogr. 2003 Sep;59(Pt 9):1632-4.
PubMed ID:12925795
44) Jormakka M, Byrne B, Iwata S.
Formate dehydrogenase--a versatile enzyme in changing environments.
Curr Opin Struct Biol. 2003 Aug;13(4):418-23.
PubMed ID:12948771
43) Jormakka M, Byrne B, Iwata S.
Protonmotive force generation by a redox loop mechanism.
FEBS Lett. 2003 Jun 12;545(1):25-30.
PubMed ID:12788488
42) Cecchini G, Maklashina E, Yankovskaya V, Iverson TM, Iwata S.
Variation in proton donor/acceptor pathways in succinate:quinone oxidoreductases.
FEBS Lett. 2003 Jun 12;545(1):31-8.
PubMed ID:12788489
41) Abramson J, Smirnova I, Kasho V, Verner G, Kaback HR, Iwata S.
Structure and mechanism of the lactose permease of Escherichia coli.
Science. 2003 Aug 1;301(5633):610-5.
PubMed ID:12893935
Related PDB Entries:1PV7, 1PV6
Press Release:
40) Horsefield R, Yankovskaya V, Tornroth S, Luna-Chavez C, Stambouli E, Barber J, Byrne B, Cecchini G, Iwata S.
Using rational screening and electron microscopy to optimize the crystallization of succinate:ubiquinone oxidoreductase from Escherichia coli.
Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):600-2.
PubMed ID:12595738
39) Yankovskaya V, Horsefield R, Tornroth S, Luna-Chavez C, Miyoshi H, Leger C, Byrne B, Cecchini G, Iwata S.
Architecture of succinate dehydrogenase and reactive oxygen species generation.
Science. 2003 Jan 31;299(5607):700-4.
PubMed ID:12560550
Related PDB Entries:1NEN, 1NEK
Press Release:
38) Iwata M, Abramson J, Byrne B, Iwata S.
Structure and function of quinone binding membrane proteins.
Adv Protein Chem. 2003;63:151-76.
PubMed ID:12629970

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37) Svensson-Ek M, Abramson J, Larsson G, Tornroth S, Brzezinski P, Iwata S.
The X-ray crystal structures of wild-type and EQ(I-286) mutant cytochrome c oxidases from Rhodobacter sphaeroides.
J Mol Biol. 2002 Aug 9;321(2):329-39.
PubMed ID:12144789
Related PDB Entries:1M57, 1M6
36) Jormakka M, Tornroth S, Byrne B, Iwata S.
Molecular basis of proton motive force generation: structure of formate dehydrogenase-N.
Science. 2002 Mar 8;295(5561):1863-8.
PubMed ID:11884747
Related PDB Entries:1KQG, 1KQF
35) Tornroth S, Yankovskaya V, Cecchini G, Iwata S.
Purification, crystallisation and preliminary crystallographic studies of succinate:ubiquinone oxidoreductase from Escherichia coli.
Biochim Biophys Acta. 2002 Jan 17;1553(1-2):171-6.
PubMed ID:11803025
34) Byrne B, Iwata S.
Membrane protein complexes.
Curr Opin Struct Biol. 2002 Apr;12(2):239-43.
PubMed ID:11959503
33) Jormakka M, Tornroth S, Abramson J, Byrne B, Iwata S.
Purification and crystallization of the respiratory complex formate dehydrogenase-N from Escherichia coli.
Acta Crystallogr D Biol Crystallogr. 2002 Jan;58(Pt 1):160-2.
PubMed ID:11752799

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32) Warkentin E, Mamat B, Sordel-Klippert M, Wicke M, Thauer RK, Iwata M, Iwata S, Ermler U, Shima S.
Structures of F420H2:NADP+ oxidoreductase with and without its substrates bound.
EMBO J. 2001 Dec 3;20(23):6561-9.
PubMed ID:11726492
Related PDB Entries:1JAY, 1JAX
31) Abramson J, Svensson-Ek M, Byrne B, Iwata S.
Structure of cytochrome c oxidase: a comparison of the bacterial and mitochondrial enzymes.
Biochim Biophys Acta. 2001 Jan 12;1544(1-2):1-9.
PubMed ID:11341911

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30) Abramson J, Larsson G, Byrne B, Puustinen A, Garcia-Horsman A, Iwata S.
Purification, crystallization and preliminary crystallographic studies of an integral membrane protein, cytochrome bo3 ubiquinol oxidase from Escherichia coli.
Acta Crystallogr D Biol Crystallogr. 2000 Aug;56(Pt 8):1076-8.
PubMed ID:10944359
29) Andreu AL, Checcarelli N, Iwata S, Shanske S, DiMauro S.
A missense mutation in the mitochondrial cytochrome b gene in a revisited case with histiocytoid cardiomyopathy.
Pediatr Res. 2000 Sep;48(3):311-4.
PubMed ID:10960495
28) Byrne B, Abramson J, Jansson M, Holmgren E, Iwata S.
Fusion protein approach to improve the crystal quality of cytochrome bo(3) ubiquinol oxidase from Escherichia coli.
Biochim Biophys Acta. 2000 Aug 15;1459(2-3):449-55.
PubMed ID:11004462
27) Abramson J, Riistama S, Larsson G, Jasaitis A, Svensson-Ek M, Laakkonen L, Puustinen A, Iwata S, Wikstrom M.
The structure of the ubiquinol oxidase from Escherichia coli and its ubiquinone binding site.
Nat Struct Biol. 2000 Oct;7(10):910-7.
PubMed ID:11017202
Related PDB Entries:1FFT
26) Sivars U, Abramson J, Iwata S, Tjerneld F.
Affinity partitioning of a poly(histidine)-tagged integral membrane protein, cytochrome bo3 ubiquinol oxidase, in a detergent--polymer aqueous two-phase system containing metal-chelating polymer.
J Chromatogr B Biomed Sci Appl.2000 Jun 23;743(1-2):307-16.
PubMed ID:10942301

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25) Andreu AL, Hanna MG, Reichmann H, Bruno C, Penn AS, Tanji K, Pallotti F, Iwata S, Bonilla E, Lach B, Morgan-Hughes J, DiMauro S.
Exercise intolerance due to mutations in the cytochrome b gene of mitochondrial DNA.
N Engl J Med. 1999 Sep 30;341(14):1037-44.
PubMed ID:10502593
24) Iwata M, Bjorkman J, Iwata S.
Conformational change of the Rieske [2Fe-2S] protein in cytochrome bc1 complex.
J Bioenerg Biomembr. 1999 Jun;31(3):169-75.
PubMed ID:10591523

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23) Iwata S, Lee JW, Okada K, Lee JK, Iwata M, Rasmussen B, Link TA, Ramaswamy S, Jap BK.
Complete structure of the 11-subunit bovine mitochondrial cytochrome bc1 complex.
Science. 1998 Jul 3;281(5373):64-71.
PubMed ID:9651245
Related PDB Entries:1BE3, 1BGY
22) Iwata S.
Structure and function of bacterial cytochrome c oxidase.
J Biochem. 1998 Mar;123(3):369-75.
PubMed ID:9538216

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21) Fushinobu S, Kamata K, Iwata S, Sakai H, Ohta T, Matsuzawa H.
Allosteric activation of L-lactate dehydrogenase analyzed by hybrid enzymes with effector-sensitive and -insensitive subunits.
J Biol Chem. 1996 Oct 11;271(41):25611-6.
PubMed ID:8810336
20) Ostermeier C, Iwata S, Michel H.
Cytochrome c oxidase.
Curr Opin Struct Biol. 1996 Aug;6(4):460-6.
PubMed ID:8794157
19) Link TA, Iwata S.
Functional implications of the structure of the 'Rieske' iron-sulfur protein of bovine heart mitochondrial cytochrome bc1 complex.
Biochim Biophys Acta. 1996 Jul 18;1275(1-2):54-60.
PubMed ID:8688452
18) Riistama S, Puustinen A, Garcia-Horsman A, Iwata S, Michel H, Wikstrom M.
Channelling of dioxygen into the respiratory enzyme.
Biochim Biophys Acta. 1996 Jul 18;1275(1-2):1-4.
PubMed ID:8688439
17) Iwata S, Saynovits M, Link TA, Michel H.
Structure of a water soluble fragment of the 'Rieske' iron-sulfur protein of the bovine heart mitochondrial cytochrome bc1 complex determined by MAD phasing at 1.5 A resolution.
Structure. 1996 May 15;4(5):567-79.
PubMed ID:8736555
Related PDB Entries:1RIE
16) Link TA, Saynovits M, Assmann C, Iwata S, Ohnishi T, von Jagow G.
Isolation, characterisation and crystallisation of a water-soluble fragment of the Rieske iron-sulfur protein of bovine heart mitochondrial bc1 complex.
Eur J Biochem. 1996 Apr 1;237(1):71-5.
PubMed ID:8620896

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15) Dohse B, Mathis P, Wachtveitl J, Laussermair E, Iwata S, Michel H, Oesterhelt D.
Electron transfer from the tetraheme cytochrome to the special pair in the Rhodopseudomonas viridis reaction center: effect of mutations of tyrosine L162.
Biochemistry. 1995 Sep 12;34(36):11335-43.
PubMed ID:7547861
14) Ostermeier C, Iwata S, Ludwig B, Michel H.
Fv fragment-mediated crystallization of the membrane protein bacterial cytochrome c oxidase.
Nat Struct Biol. 1995 Oct;2(10):842-6.
PubMed ID:7552705
Related PDB Entries:1MQK
13) Iwata S, Ostermeier C, Ludwig B, Michel H.
Structure at 2.8 A resolution of cytochrome c oxidase from Paracoccus denitrificans.
Nature. 1995 Aug 24;376(6542):660-9.
PubMed ID: 7651515
Related PDB Entries:1QLE
12) Kleymann G, Iwata S, Wiesmuller KH, Ludwig B, Haase W, Michel H.
Immunoelectron microscopy and epitope mapping with monoclonal antibodies suggest the existence of an additional N-terminal transmembrane helix in the cytochrome b subunit of bacterial ubiquinol:cytochrome-c oxidoreductases.x
Eur J Biochem. Wiesmuller KH, Ludwig B, Haase W, Michel H.
PubMed ID:7601123

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11) Iwata S, Kamata K, Yoshida S, Minowa T, Ohta T.
T and R states in the crystals of bacterial L-lactate dehydrogenase reveal the mechanism for allosteric control.
Nat Struct Biol. 1994 Mar;1(3):176-85.
PubMed ID:7656036
Related PDB Entries:1LTH
10) Iwata S, Yoshida S, Ohta T.
A regular 1:1 complex of two allosteric states in the single crystal of L-lactate dehydrogenase from Bifidobacterium longum.
J Mol Biol. 1994 Feb 25;236(3):958-9.
PubMed ID:8114106
Related PDB Entries:1LTH

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9) Tanokura M, Sasaki H, Muramatsu T, Iwata S, Hamaya T, Takizawa T, Takahashi K.
A new crystal form of proteinase A, a non-pepsin-type acid proteinase from Aspergillus niger var. macrosporus.
J Biochem. 1993 Oct;114(4):457-8.
PubMed ID:8276753
8) Iwata S, Ohta T.
Molecular basis of allosteric activation of bacterial L-lactate dehydrogenase.
J Mol Biol. 1993 Mar 5;230(1):21-7.
PubMed ID:
Related PDB Entries:1LLD

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7) Tanokura, M., Matsuzaki, H., Iwata, S., Nakagawa, A., Hamaya, T., Takizawa, T., and Takahashi, K.
Effect of dimethylsulfoxide on the crystallization of Aspergillus niger proteinase A.
Proc. Japan Acad. Sci. 1992; 67B:209-212
6) Tanokura M, Matsuzaki H, Iwata S, Nakagawa A, Hamaya T, Takizawa T, Takahashi K.
Crystallization and preliminary X-ray investigation of proteinase A, a non-pepsin-type acid proteinase from Aspergillus niger var. macrosporus.
J Mol Biol. 1992 Jan 5;223(1):373-5.
PubMed ID:1731082
5) Ohta T, Yokota K, Minowa T, Iwata S.
Mechanism of allosteric transition of bacterial L-lactate dehydrogenase.
Faraday Discuss. 1992;(93):153-62.
PubMed ID:1290930

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4) Koide S, Iwata S, Matsuzawa H, Ohta T.
Crystallization of allosteric L-lactate dehydrogenase from Thermus caldophilus and preliminary crystallographic data.
J Biochem. 1991 Jan;109(1):6-7.
PubMed ID:2016275

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3) Iwata S, Minowa T, Mikami B, Morita Y, Ohta T.
Crystallization of and preliminary crystallographic data for allosteric L-lactate dehydrogenase from Bifidobacterium longum.
J Biochem. 1989 Oct;106(4):558-9.
PubMed ID:2691505
2) Minowa T, Iwata S, Sakai H, Masaki H, Ohta T.
Sequence and characteristics of the Bifidobacterium longum gene encoding L-lactate dehydrogenase and the primary structure of the enzyme: a new feature of the allosteric site.
Gene. 1989 Dec 21;85(1):161-8.
PubMed ID:2695396
1) Iwata, S., Minowa, T., Sakai, H. and Ohta, T.
Amino acid residues in the allosteric site of L-lactate dehydrogenase from Bifidobacterium longum.
Agril. and Bio. Chem. 1989; 53: 3365-3366

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